e faecium strain atcc 8459  (ATCC)

Journal: Poultry Science

Article Title: Applying microbial predictive and machine learning model data to evaluate thermal inactivation of salmonella and the surrogate enterococcus faecium in reconstructed ground chicken meat affected by temperature and salt concentrations

doi: 10.1016/j.psj.2025.105422

Figure Lengend Snippet: Decrease of Enterococcus faecium in moisture enhanced reconstructed ground chicken with 0, 1, 3, and 5 % of salt over time (s) in response to temperature treatments at 62, 66, 70, and 74°C in a circulated thermal water bath.

Article Snippet: Thermal processing studies have shown that a minimum extrusion temperature of 74°C in a balanced carbohydrate-protein meal can achieve a 5-log reduction of E. faecium strain ATCC 8459.

Techniques:

Journal: Poultry Science

Article Title: Applying microbial predictive and machine learning model data to evaluate thermal inactivation of salmonella and the surrogate enterococcus faecium in reconstructed ground chicken meat affected by temperature and salt concentrations

doi: 10.1016/j.psj.2025.105422

Figure Lengend Snippet: Decrease of Enterococcus faecium (Log 10 CFU) in moisture enhanced reconstructed ground chicken with 0, 1, 3, and 5 % of salt over time (s) in response to temperature treatments at 62, 66, 70, and 74°C in a circulated thermal water bath. Lines through data show predicted Log 10 CFU from model that included an orthogonal polynomial of time in sec, NaCl concentrations, and water bath temperatures. Dashed lines show the thermal inactivation threshold for each treatment. Estimated d -values indicated in boxes on each panel.

Article Snippet: Thermal processing studies have shown that a minimum extrusion temperature of 74°C in a balanced carbohydrate-protein meal can achieve a 5-log reduction of E. faecium strain ATCC 8459.

Techniques:

Journal: BMC Biology

Article Title: The quorum sensing peptide EntF* promotes colorectal cancer metastasis in mice: a new factor in the host-microbiome interaction

doi: 10.1186/s12915-022-01317-z

Figure Lengend Snippet: In vitro formation and in vivo presence of the EntF* metabolite. a Sequences of the enterocin induction factor pro-peptide, mature quorum sensing peptide EntF, and its metabolite EntF*. b In vitro formation rate of EntF* from EntF in colon ( n = 7) and feces ( n = 4) homogenates. Bars represent the mean formation rate ± SEM from independent experiments. Statistically significant differences were determined by a Mann-Whitney U test with indicated p -values. c Apparent permeability coefficients ( P app ) of PapRIV, EntF*, and EDF-analog in Caco-2 cells. Bars represent mean P app values ± SEM ( n = 6 independent experiments); the shaded area represents the limit of detection. d Flow chart displaying the experimental design stages, from serum sampling to peptide detection and further confirmation of EntF* presence in vivo. Different LC-MS methods: LC 1 -MS 1 , reversed-phase ultra-high-performance liquid chromatography (RP-UPLC) using triple quadrupole (TQ) in MRM mode; LC 1 -MS 2 , high-resolution quadrupole time-of-flight; LC 1 -MS 3 , high-resolution quadrupole-orbitrap; LC 2 -MS 1 , HILIC-amide UPLC using TQ in MRM mode. qPCR was performed on feces sample of mice from the same set to demonstrate the presence of EntF-encoding DNA sequences from E. faecium . e Chromatographic profiles of (1) negative serum sample, (2) positive serum sample, (3) serum sample from EntF*-treated mice. Chromatographic profiles were obtained using RP-UPLC with detection by electrospray ionization mass spectrometry (ESI-MS) using TQ in MRM mode ( m/z = 865 ➔ 202.08 + 315.17). f Chromatographic profiles of (1) negative serum sample, (2) positive serum sample, (3) serum sample from EntF*-treated mice. Chromatographic profiles were obtained using HILIC amide UPLC with detection by ESI-MS using TQ in MRM mode ( m/z = 865 ➔ 202.08 + 315.17). g Isotopic distribution of the double charged EntF* measured in a positive serum sample using RP-UPLC with detection by ESI-MS using quadrupole-orbitrap. h High-resolution tandem mass spectrum of EntF* with characteristic fragments, using RP-UPLC with detection by Q-TOF. i In vivo presence of EntF* in gnotobiotic mice treated with EntF-producing bacterial strains. Number of EntF DNA copies per gram of feces measured four days after treatment with placebo (300 μL BHI medium) (limit of detection: 10 5 copies/g) (left). EntF* concentration in colon content. No EntF* was detected in the placebo group (the red line indicates the limit of detection) (middle). EntF* concentration in serum content. No EntF* was detected in the placebo group (the red line indicates the limit of detection) (right)

Article Snippet: At day 0, the placebo group was treated with the cell medium (BHI), the control group received a mixture of 3 EntF-negative E. faecium strains (NCIMB 10415; W54 and LMG S-28935 each 10 8 CFU per strain) suspended in 300 μL of BHI, while the test group received a mixture of 3 EntF-producing E. faecium strains (LMG 23236; T-110 and ATCC 8459 each 10 8 CFU/strain) suspended in 300 μL of BHI.

Techniques: In Vitro, In Vivo, MANN-WHITNEY, Permeability, Sampling, Liquid Chromatography with Mass Spectroscopy, High Performance Liquid Chromatography, Targeted Proteomics, Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry, Concentration Assay